ABSTRACT
Objective:To establish a determination method for loganin in Tiaojing Zhuyun granules by HPLC. Methods: HPLC was used with a SinoChrom ODS-BP (250 mm × 4. 6 mm,5μm) as the analytical column. The mobile phase was composed of acetoni-trile-0. 1% phosphoric acid solution(14∶86). The detection wavelength was set at 240 nm and the column temperature was 30℃. Re-sults:The calibration curve of loganin was in a good linearity over the range of 50. 40-1 008. 00 ng(r=0. 999 9). The average recov-ery was 99. 33%(RSD=1. 41%, n=6). Conclusion:The method is simple,feasible and reproducible,and can be used in the quality control of Tiaojing Zhuyun granules.
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Objective:To establish the fingerprint analysis method for the aqueous extracts of Eucommia ulmoides from Enshi by HPLC. Methods:The fingerprint of aqueous extracts of ten batches of Eucommia ulmoides from Enshi were analyzed by HPLC. The columnwasWondaSilC18(250mm×4.6mm,5μm). Themobilephaseconsistedofacetonitrile-0.1% phosphoricacidwithgradient elution. The flow rate was 1. 0 ml·min-1 , the detection wavelength was 230 nm, the column temperature was 25℃, and the injection volume was 10 μl. Results:The fingerprint consisted of 12 common peaks. The similarity range of ten batches of Eucommia ulmoides calculated by similarity evaluation system for the chromatographic fingerprint of TCM(Version 2004 A)was 0. 596-0. 997. The standard fingerprint of Eucommia ulmoides was established by HPLC. Conclusion: The established HPLC fingerprint analysis method for Eu-commia ulmoides from Enshi is simple, stable and reproducible, which can effectively control the quality of Eucommia ulmoides from Enshi.
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Objective:To optimize β-cyclodextrin (β-CD) inclusion process for volatile oil in Yingxinning capsules. Methods:The content of volatile oil in theβ-CD inclusion compound as the evaluation index, the ratio of volatile oil toβ-CD, inclusion tempera-ture, inclusion time and stirring speed as the influencing factors, the inclusion process was studied using L9(34)orthogonal design. Re-sults:The best preparation conditions were as follows:the ratio of volatile oil toβ-CD was 1∶ 9, the inclusion temperature was 40℃, the inclusion time was 30min and the stirring speed was 800 r·min-1 . Conclusion: The preparation technology is simple, feasible and stable with high inclusion rate of volatile oil.
ABSTRACT
ThestudiesonthemaincomponentsinEucommiaulmoidesfromdomesticandabroadweresummarizedinthepaper, which can provide the reference for the effective component study and quality control of the Chinese herb.